Exploration of Immunology

Table 1. Summary of the comparison between ISTH and CLSI guidelines for LA detection

Category	ISTH 2020	CLSI 2014
Sample preparation	Double centrifugation	Double centrifugation
Assay to use	dRVVT and APTT	dRVVT and APTT and/or others
Testing order	Screening => mixing & confirmatory	Screening => confirmatory => mixing
Calculations for PL-dependence	Normalized ratio or percentage correction	Normalized ratio or percentage correction
Mixing test	Perform on: mixture with NPP Interpret with MTC	Perform on: mixture with NPP Interpret with MTC or ICA

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MTC: mixing test-specific cut-off; ICA: index of circulating anticoagulant

Declarations

Author contributions

OK: Conceptualization, Investigation, Writing—original draft, Writing—review & editing. MP: Investigation, Writing—review & editing. JA: Supervision, Validation, Writing—review & editing. All authors read and approved the submitted version.

Conflicts of interest

OK was an employee of HYPHEN BioMed at the time the work carried out. MP is an employee of HYPHEN BioMed. JA is a consultant for HYPHEN BioMed and Sysmex Corporation.

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

Not applicable.

Funding

Not applicable.

Copyright

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