Exploration of Immunology

Laboratory testing	Assay principle	Measurement type
Functional assays	Inhibition of activity	Measurement of factor activity; mixing studies.
Titration of inhibitor titer (Bethesda method)	Incubation of a normal sample with serial dilutions of patient sample and testing residual activity	Dilution inhibiting 50% of activity (corresponds to 1 unit)
Flow cytometry	Detection of circulating autoantibodies through their binding to Plt or blood cell GPs	Detection of bound antibodies to specific GPs
ELISA	Autoantigen capture-based assays	Detection of autoantibody by ELISA
MAIPA	Immunocapture of Plt GP and reactive autoantibodies	Identification of specific anti-GP autoantibodies
Coombs test	Detection of cell-bound antibodies	Detection of global anti-cell autoantibodies

Laboratory testing

Assay principle

Measurement type

Functional assays

Inhibition of activity

Measurement of factor activity; mixing studies.

Titration of inhibitor titer (Bethesda method)

Incubation of a normal sample with serial dilutions of patient sample and testing residual activity

Dilution inhibiting 50% of activity (corresponds to 1 unit)

Flow cytometry

Detection of circulating autoantibodies through their binding to Plt or blood cell GPs

Detection of bound antibodies to specific GPs

ELISA

Autoantigen capture-based assays

Detection of autoantibody by ELISA

MAIPA

Immunocapture of Plt GP and reactive autoantibodies

Identification of specific anti-GP autoantibodies

Coombs test

Detection of cell-bound antibodies

Detection of global anti-cell autoantibodies