Primer sequences were used for reverse transcription-polymerase chain reaction (RT-PCR) and to discriminate human factor H (FH), FH-like-1 (FHL-1), and FH-related protein-1 (FHR-1)
| Primer name | Sequence | mRNA target |
|---|---|---|
| FH short consensus repeat 2 (SCR2) | Forward: AGGCCCTGTGGACATCReverse: AACTTCACATATAGGAATATC | FH and FHL-1, across exons 3–4, SCR2 (5’ end) |
| FH exon 9/11 | Forward: CTACTCCCAGATGCATCCGTGTReverse: GGTTGAGCTGACCATCCATCTTT | FH, across exons 9–11 (middle) |
| FH SCR20 | Forward: CATCCGTGTGTAATATCCCGReverse: TCTTTTTGCACAAGTTGGATAC | FH, across exons 22–23, SCR20 (3’ end); potentially FHR-1 across exons 5–6 |
| FHL-1 | Forward: CATCCGTGTCAGCTTTACCCTReverse: CCATTCTGGCTGGAATAATACAC | FHL-1 unique 4 amino acid tail |
| FHR-1 | Forward: GTACTGTGGGCGGATTCACAReverse: CTCTGTTGGGGGAGAAGAAAAATA | FHR-1 A and B, exons 1–4 |
| Cyclophilin | Forward: GGCAAATGCTGGACCCAACACAAAReverse: CTAGGCATGGGAGGGAACAAGGAA | Cyclophilin A, housekeeping gene |