IL-6 SNP rs1800795 primers and probes
| SNP rs1800795 | |
|---|---|
| Primers | Forward: 5’-CGACCTAAGCTGCACTTTTCC-3’ |
| Reverse: 5’-GGGCTGATTGGAAACCTTATTAAGATTG-3’ | |
| Probes | 5’-CCTTTAGCATGGCAAGAC-3’ labelled with VIC |
| 5’-CCTTTAGCATCGCAAGAC-3’ labelled with FAM | |
The PCR program used for amplification: 1 cycle, 2 min at 50°C; 1 cycle, 10 min at 95°C (initial denaturation); 40 cycles, 15 sec at 95°C (denaturation), 1 min at 60°C (extension). IL-6: interleukin-6; SNP: single nucleotide polymorphism