Exploration of Neuroprotective Therapy

From: Role of dopamine receptor heteromerization in preclinical models of addiction and other psychiatric disorders

Table 1

Main techniques to assess receptor heteromerization

Method	Proximity/resolution	Sample type
Co-IP	N.A./sampled tissue	Lysed tissue
In situ hybridization	> 500 nm–1 µm	Heterologous system/native tissue
Immunogold	20–30 nm	Native tissue
PLA	16 nm (strand level)–40 nm (epitope level)	Native tissue/heterologous system
BRET	< 10 nm	Heterologous system
FRET	< 10 nm	Heterologous system
TR-FRET	< 10 nm	Heterologous system/native tissue/membrane preparations
SRET	< 10 nm	Heterologous system
AlphaLisa	< 200 nm	Membrane preparations
NanoBiT	N.D./low molecular weight of NanoLuc (19 kDa)	Heterologous system
SPT	100–300 nm Movement detection < 25 nm	Heterologous system/culture of neurons

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N.A.: not applicable; N.D.: not determined; Co-IP: co-immunoprecipitation; PLA: proximity ligation assay; BRET: bioluminescence resonance energy transfer; FRET: fluorescence/Förster resonance energy transfer; TR-FRET: time-resolved FRET; SRET: sequential resonance energy transfer; SPT: single particle tracking