From:  Blockage of thrombospondin 4 secreted by spinal astrocytes may be a promising therapeutic target in the treatment of neuropathic pain

Inhibition or blockage of TSP4 expression through TSP4-AON or antibody reversed or prevented the development of behavioral hypersensitivity

StudyAnimals (species/types)ProceduresResults
Kim et al. [41], 2012Male young adult Harlan Sprague-Dawley ratsTSP4 antibody 80 μg/rat, intrathecal, two weeks after L5/6 SNLBoth tactile allodynia and mechanic and thermal hyperalgesia reversed
All effects peaked at 6 h after TSP4 antibody injection
TSP4 antibody, 80 μg/rat per day, intrathecal immediately before, for eight days, and after SNLPre-emptive TSP4 antibody treatment prevented allodynia onset
TSP4-AON, 50 μg/rat per day, intrathecal, for four days after SNLTactile allodynia and thermal and mechanical hyperalgesia reversed time-dependently
Zeng et al. [98], 2013Adult female Sprague-Dawley ratsTSP4-AON, 50 μg/rat per day, intrathecal, post-SCI for four daysThe antiallodynic effect developed after the last injection and lasted more than two days
SCI-induced TSP4 expression was reduced in the DSC by 40%
Li et al. [97], 2014Adult male Sprague-Dawley ratsTSP4-AON, 50 μg/10 μL per rat per day, intrathecal, for four consecutive days, starting at least three weeks post-CCI of the infraorbital nerveAfter the last injection, the antiallodynic effect peaked at approximately one day and lasted over two days. Simultaneously, injury-induced TSP4 expression diminished in the C1/2 DSC
Crosby et al. [30], 2015Adult male Holtzman ratsTSP4-AON, 75 μg/rat per day, intrathecal, for three days before FJD and seven days after FJDPre-emptive TSP4-AON treatment prevented the development of tactile allodynia and the increase in TSP4 expression in the C6/7 spinal cord following FJD
FJD-induced excitatory synapse density is reduced in the superficial DH
Return to article view