Description of dyes for MitoTracker Green FM and MitoTracker Deep Red FM
| Dye | Manufacturer | Target organelle | Excitation wavelength | Emission wavelength | Stock | Dilution used |
|---|---|---|---|---|---|---|
| MitoTracker Green FM | Thermo Fischer Scientific (catalog No. M7514) | Mitochondria | 490 nm | 516 nm | 50 μg in 1 mL DMSO | 300 nM (diluted in pre-warmed DMEM) |
| MitoTracker Deep Red FM | Thermo Fischer Scientific (catalog No. M22426) | Mitochondria | 644 nm | 665 nm | 50 μg in 1 mL DMSO | 500 nM (diluted in pre-warmed DMEM) |
DMEM: Dulbecco’s modified eagle medium; DMSO: dimethylsulfoxide