Ease of application of drugs dissolved in the embryonic media in embryos and early larvae

Ease of liver imaging in intact organism

Differences in the liver architecture between zebrafish and humans may alter pathogenesis of DILI species-specifically

Ability to screen a high number of biological replicates using a wide range of doses

Most GWAS loci are non-coding and may not be well conserved between species

Availability of various mutant strains of GWAS loci as ready-to-use models

Speedy generation of knockout and transgenic strains

Difficulty in functional assessment of knock-out and knock-in strains because of the presence of paralogous copies of genes in zebrafish (one-to-many genes)

Presence of many GWAS human loci with orthologs in the zebrafish genome

Not all human GWAS loci have orthologs in zebrafish

Observed similarity between humans and zebrafish in their pathogenic response to drugs

Varying levels of sequence similarity between protein coding genes of zebrafish and humans

Potential for generating specialized zebrafish cohorts for eQTL studies

Requirement of a large collection of strains/individuals for idiosyncratic DILI studies in zebrafish