Exploration of Digestive Diseases

Table 1

Human immunophenotyping standard panels. This table lists the combination of markers frequently used to analyze different immune cells populations

Parental populations	Parental markers	Subpopulation	Subpopulation markers	Ref.
CD4 T cells	CD3⁺/CD20^–/CD8⁻/CD4⁺	Naive	CCR7⁺/CD45RA⁺	[18, 19]
		Central memory	CCR7⁺/CD45RA⁻
		Terminal effector	CCR7⁻/CD45RA⁺
		Effector memory	CCR7⁻/CD45RA⁻
		Treg	CD127^low/CD25^Hi/CCR4⁺
		Th1	CD25^low/−/CXCR5⁻/CD45RA⁻/CXCR3⁺/CCR6⁻	[19]
		Th2	CD45RA^–/CCR4⁺/CCR6^–/CXCR3⁺	[20]
		Th1Th17	CD45RA^–/CCR4^–/CCR6⁺/CXCR3⁺	[20]
		Th17	CD25^low/−/CXCR5⁻/CD45RA⁻/CXCR3⁻/CCR6⁺	[19]
		Th22	CD194⁺/CD196⁺/CCR10⁺	[21]
CD8 T cells	CD3⁺/CD20^–/CD8⁺/CD4^–	Naive	CCR7⁺/CD45RA⁺	[18, 19, 22]
		Central memory	CCR7⁺/CD45RA⁻
		Terminal effector	CCR7⁻/CD45RA⁺
		Effector memory	CCR7⁻/CD45RA⁻
Unconventional T cells	CD3⁺/CD20^–	NKT cells	TCRVα24JαQ⁺	[19]
		MAIT cells	CD161⁺/TCRVα7.2⁺
		γδ T cells	TCRVαβ⁻/TCRVγδ⁺
NK cells	CD3⁻/CD56⁺	-	-	[19]
Granulocytes	CD45⁺/CD3^–/CD19^–	Neutrophils	CD16⁺	[23]
		Basophils	CD123⁺/HLA-DR^–
		Eosinophils	CD16^–
B cells	CD3^–/CD20⁺	Transitional	CD10⁺/CD27⁻	[19]
		Naive	CD27⁻/CD10⁻
		Memory	CD27⁺/CD10⁻
Monocytes	CD45⁺/CD3^–/CD19^–/CD123^–/HLA-DR⁺/CD11c⁺	Classical	CD14⁺CD16^–	[23]
		Intermediate	CD14⁺CD16⁺
		Non-classical	CD14^–CD16⁺
Dendritic cells	CD45⁺/CD3^–/CD19^–	Myeloid	CD123^–CD11c⁺/CD16^–CD14^–/HLA-DR⁺⁺	[23]
Dendritic cells	CD45⁺/CD3^–/CD19^–	Plasmacytoid	CD123⁺/HLA-DR⁺	[23]

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CCR7: C-C chemokine receptor type 7; CXCR5: C-X-C motif chemokine receptor 5; HLA: human leucocyte antigen; MAIT: mucosal-associated invariant T; Th1: T helper 1; Treg: regulatory T cell; -: none