Summary of validation parameters for HPLC method
| Validation parameters | OXY | PMB |
|---|---|---|
| Absorption maxima (λ max; nm) | 303 | |
| Linearity and range (μg/mL) | 5–25 | |
| Coefficient of determination (R2) | 0.9915 | 0.9961 |
| Regression equation (y) | 3,280.7x – 9,906.6 | 2,437.6x – 10,926 |
| Slope (m) | 3,280.7 | 2,437.6 |
| Intercept (c) | 9,906 | 10,926 |
| LOD (μg/mL) | 0.197 | 0.173 |
| LOQ (μg/mL) | 0.599 | 0.525 |
| Precision | 1.21 | 0.89 |
| Robustness | 1.569 | 0.879 |