Characterization and cytotoxic effect of precursor peptides in HeLa and Ca Ski cells after 2 h treatment at 37°C
| Functional peptide | Sequence | RP-HPLC | ESI-MS (M) | IC50 (μM) | |||
|---|---|---|---|---|---|---|---|
| tR (min) | Purity (%) | Theorical | Experimental | HeLa | Ca Ski | ||
| Anticancer peptides (ACP) | RWQWRWQWR | 6.6 | 98.1 | 1,485.76 | 1,485.78 | 47.6 | > 134.5 |
| Ahx-RWQWRWQWR | 6.5 | 97.6 | 1,598.85 | 1,598.88 | 103.3 | > 125.0 | |
| RWQWRWQWR-Ahx | 7.1 | 98.6 | 1,598.85 | 1,598.86 | > 125.0 | > 125.0 | |
| RRWQWR | 5.3 | 99.3 | 985.55 | 985.58 | > 202.0 | > 202.0 | |
| Ahx-RRWQWR | 5.4 | 97.8 | 1,098.63 | 1,098.65 | > 181.9 | > 181.9 | |
| RLLRRLLR | 5.1 | 97.8 | 1,093.77 | 1,093.79 | > 182.7 | > 182.7 | |
| Ahx-RLLRRLLR | 5.9 | 97.3 | 1,206.85 | 1,206.87 | > 165.6 | > 165.6 | |
| Cervical cancer cell-targeting peptides (CTP) | QQLPSSSTSTYP | 4.0 | 90.7 | 1,293.62 | 1,293.65 | > 154.5 | > 154.5 |
| Ahx-QQLPSSSTSTYP | 4.1 | 96.5 | 1,406.70 | 1,406.72 | > 142.1 | > 142.1 | |
| GDALFSVPLEVY | 7.1 | 98.7 | 1,307.68 | 1,308.20 | > 152.8 | > 152.8 | |
| Ahx-GDALFSVPLEVY | 7.2 | 98.2 | 1,420.76 | 1,420.78 | > 140.7 | > 140.7 | |
| QVNGLGERSQQM | 3.8 | 86.2 | 1,344.66 | 1,344.67 | > 148.6 | > 148.6 | |
| Ahx-QVNGLGERSQQM | 4.0 | 93.1 | 1,457.74 | 1,457.76 | > 137.1 | > 137.1 | |
| KQNLAEG | 3.8a | 97.2 | 757.41 | 757.42 | > 263.9 | > 263.9 | |
| Ahx-KQNLAEG | 4.4a | 97.0 | 870.49 | 870.52 | > 229.6 | > 229.6 | |
| Cell-penetrating peptides (CPP) | YGRKKRPQRRR | 3.8a | 99.2 | 1,498.92 | 1,498.92 | > 133.4 | > 133.4 |
| Ahx-YGRKKRPQRRR | 4.4a | 94.5 | 1,612.00 | 1,612.04 | > 124.0 | > 124.0 | |
| RRRRRRRR | 4.0a | 96.7 | 1,265.84 | 1,265.84 | > 157.9 | > 157.9 | |
| Ahx-RRRRRRRR | 4.2a | 95.6 | 1,378.92 | 1,378.94 | > 145.0 | > 145.0 | |
| RKKRRQRRR | 3.7a | 93.8 | 1,337.88 | 1,337.90 | > 149.4 | > 149.4 | |
| Ahx-RKKRRQRRR | 3.8a | 98.2 | 1,450.97 | 1,450.98 | > 137.8 | > 137.8 | |
| KLALKLALK | 5.6 | 92.4 | 995.72 | 995.73 | > 200.7 | > 200.7 | |
| Ahx-KLALKLALK | 5.8 | 95.5 | 1,108.81 | 1,108.81 | > 180.3 | > 180.3 | |
a: RP-HPLC modified linear gradient (method 2). tR: retention time; Ahx: 6-amino hexanoic acid; RP-HPLC: reverse phase-high performance liquid chromatography; ESI-MS: electrospray ionization-mass spectrometry. All peptides were obtained with amide function at the C-terminal end