Characterization and cytotoxic effect of hybrid peptides in HeLa, Ca Ski, and L929 cells after 2 h treatment at 37°C
| Code | Sequence | RP-HPLC | ESI-MS (M) | IC50 (μM) | ||||
|---|---|---|---|---|---|---|---|---|
| tR (min) | Purity (%) | Theorical | Experimental | HeLa | Ca Ski | L929 | ||
| 1 | RWQWRWQWR-Ahx-RRWQWR | 7.0 | 96.1 | 2,567.37 | 2,567.40 | 21.6 | 28.6 | 40.2 |
| 2 | RRWQWR-Ahx-RWQWRWQWR | 6.9 | 96.3 | 2,567.37 | 2,567.37 | 26.7 | 48.9 | 55.5 |
| 3 | RWQWRWQWR-Ahx-RLLRRLLR | 7.8 | 97.8 | 2,675.59 | 2,675.61 | 13.3 | 16.8 | 30.7 |
| 4 | RLLRRLLR-Ahx-RWQWRWQWR | 7.0 | 97.4 | 2,675.59 | 2,675.60 | 16.0 | 22.4 | 42.4 |
| 5 | RWQWRWQWR-Ahx-QQLPSSSTSTYP | 7.0 | 97.7 | 2,875.44 | 2,875.46 | > 69.5 | ND | ND |
| 6 | RWQWRWQWR-Ahx-GDALFSVPLEVY | 8.3 | 99.3 | 2,889.50 | 2,889.55 | > 69.2 | ND | ND |
| 7 | RWQWRWQWR-Ahx-QVNGLGERSQQM | 7.0 | 98.2 | 2,926.48 | 2,926.52 | > 68.3 | ND | ND |
| 8 | RWQWRWQWR-Ahx-KQNLAEG | 6.8 | 99.2 | 2,339.23 | 2,339.25 | > 85.4 | ND | ND |
| 9 | RWQWRWQWR-Ahx-YGRKKRPQRRR | 6.5 | 99.1 | 3,080.74 | 3,080.76 | 19.4 | 34.3 | 23.6 |
| 10 | RWQWRWQWR-Ahx-RRRRRRRR | 6.4 | 98.5 | 2,847.66 | 2,847.70 | 12.8 | 55.5 | 22.3 |
| 11 | RWQWRWQWR-Ahx-RKKRRQRRR | 6.3 | 98.3 | 2,919.70 | 2,919.72 | 17.2 | 67.7 | 25.0 |
| 12 | RKKRRQRRR-Ahx-RWQWRWQWR | 6.4 | 97.3 | 2,919.70 | 2,919.68 | 17.1 | 16.4 | 20.2 |
| 13 | RWQWRWQWR-Ahx-KLALKLALK | 7.5 | 98.5 | 2,577.54 | 2,577.60 | 12.0 | 71.7 | > 77.5 |
| 14 | KLALKLALK-Ahx-RWQWRWQWR | 7.2 | 99.2 | 2,577.54 | 2,577.54 | 15.1 | 34.6 | 30.9 |
| 15 | KWQWKWQWK-Ahx-RRWQWR | 6.8 | 91.8 | 2,483.35 | 2,483.38 | 14.6 | 19.6 | 6.7 |
| 16 | RWQWRWQWR-Ahx-KKWQWK | 6.9 | 98.4 | 2,483.35 | 2,483.38 | 15.7 | 74.6 | > 80.5 |
| 17 | KWQWKWQWK-Ahx-KKWQWK | 6.7 | 98.2 | 2,399.33 | 2,399.36 | 23.9 | 35.1 | 16.5 |
| 18 | (RWQWRWQWR)2K-Ahx-RRWQWR | 7.3 | 93.1 | 4,164.2 | 4,164.24 | 9.6 | 36.0 | 35.6 |
ND: not determined; Ahx: 6-aminohexanoic acid (magenta); RP-HPLC: reverse phase-high performance liquid chromatography; ESI-MS: electrospray ionization-mass spectrometry