Dimeric peptides characterization by RP-HPLC and LC-MS
| Code | Sequence | RP-HPLC | Monoisotopic mass | ||
|---|---|---|---|---|---|
| tR (min) | Purity (%) | Theoretical | Experimental | ||
| 26[F] | (RRWQWRFKKLG)2-K-Ahx | 5.9 | 99 | 3,341.964 | 3,341.9736 |
| 1 | (dRRWQWRFKKLG)2-K-Ahx | 6.0 | 88 | 3,341.964 | 3,342.2998 |
| 2 | (Orn-RWQWRFKKLG)2-K-Ahx | 5.9 | 84 | 3,257.995 | 3,258.7513 |
| 3 | (R-Orn-WQWRFKKLG)2-K-Ahx | 5.8 | 90 | 3,257.995 | 3,257.9099 |
| 4 | (RRWQW-Orn-FKKLG)2-K-Ahx | 5.8 | 99 | 3,257.995 | 3,257.7685 |
| 5 | (dR-Orn-WQWRFKKLG)2-K-Ahx | 5.9 | 84 | 3,257.995 | 3,256.8235 |
| 6 | (dRRWQW-Orn-FKKLG)2-K-Ahx | 5.9 | 86 | 3,257.995 | 3,257.8924 |
| 7 | (Ahx-RRWQWRFKKLG)2-K-Ahx | 5.9 | 83 | 3,568.132 | 3,568.1213 |
| 8 | (Biotin-Ahx-RRWQWRFKKLG)2-K-Ahx | 6.1 | 90 | 4,021.452 | 4,020.2752 |
| 9 | (CH3CO-Ahx-RRWQWRFKKLG)2-K-Ahx | 6.3 | 86 | 3,652.142 | 3,652.1590 |
| 10 | (RWQWRWQWR)2-K-Ahx | 7.2 | 99 | 3,195.670 | 3,196.6716 |
| 11 | (Ahx-RWQWRWQWR)2-K-Ahx | 7.1 | 99 | 3,422.838 | 3,422.8376 |
| 12 | (RWQWRWQW-Orn)2-K-Ahx | 7.2 | 97 | 3,111.642 | 3,111.6287 |
| 13 | (CH3CO-RWQWRWQWR)2-K-Ahx | 7.4 | 91 | 3,280.713 | 3,280.6926 |
LC-MS: liquid chromatography-mass spectrometry; RP-HPLC: reverse phase-high performance liquid chromatography; tR: retention time