Results for the Irwin test. Mice administered with peptide 3 at a dose of 70 mg/kg (group 1; n = 3) or 140 mg/kg (group 2; n = 3)
| Item | Group 1 | Group 2 | ||||
|---|---|---|---|---|---|---|
| < 5 min | 15 min | > 30 min | < 5 min | 15 min | > 30 min | |
| Autonomic | ||||||
| Salivation | ||||||
| Lachrymation | ||||||
| Piloerection | ↑ | |||||
| Excessive urine | ||||||
| Diarrhea | ||||||
| Abnormal breathing | ↓↓ | ↓↓↓ | ||||
| Neuromuscular | ||||||
| Abnormal posture | ↓↓ | ↓↓ | ↓↓ | ↓↓ | ↓↓↓ | |
| Muscular tone | ↓↓ | ↓↓ | ↓↓ | ↓↓ | ↓↓↓ | |
| Tremors | ||||||
| Seizures | ||||||
| Catalepsy | ||||||
| Straub’s tail | ||||||
| Exophthalmos | ||||||
| Motor-sensory | ||||||
| Touch response | ↓ | ↓ | ↓↓ | ↓↓↓ | ↓↓↓ | |
| Pinna reflex | ||||||
| Tensile response | ↓ | ↓ | ↓ | ↓↓ | ↓↓↓ | |
| Behavioral | ||||||
| Sedation | ↑ | ↑↑ | ↑↑ | ↑↑ | ↑↑↑ | |
| Reactivity to handling | ↓ | ↓↓ | ↓↓ | ↓↓ | ↓↓↓ | |
| Spontaneous activity | ↓↓ | ↓↓ | ↓↓ | ↓↓ | ↓↓↓ | |
| Scratching | ↑↑↑ | ↑↑↑ | ||||
| Dead | ||||||
In the Irwing test, differences were observed in the evaluated items compared to the control group (injection with saline solution) (n = 2). The arrow ↑ indicated an increase in the effect and ↓ a decrease. The intensity of the effect was evaluated by the number of arrows present (for example: ↓: mild intensity; ↓↓: moderate intensity; ↓↓↓: high intensity). The colors represent the number of individuals in each group that exhibited the behavior; pink (none), deep pink (one), red (two), and deep red (three)