From:  Development of solid lipid nanoparticle gel for transdermal delivery system of chaulmoogra oil
 

Validation parameters of developed HPTLC method of HA (n = 6)

Linearity parametersObservation
Marker quantifiedHA
Solvent systemHexane:ethyl acetate (8:1, v/v)
Wavelength645 nm
Rf obtained0.45
Linearity range (ng/spot)200–2,000
Regression equationY = 986.533 + 8.281x
Correlation coefficient ± SD0.9930 ± 0.0005
Slope ± SD8.281 ± 0.6
Intercept ± SD986.533 ± 1.84
Accuracy (recovery)97–103%
Limit of detection (ng)10
Limit of quantitation (ng)25
Precision (%RSD)0.64–1.82
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SD: standard deviation; RSD: relative standard deviation