Exploration of Medicine

Table 1

Retention time (Rt) (min), maximum absorption in the visible region (λ_max) (nm), deprotonated ion ([M-H]) (m/z), mass fragmentation (MS²) (m/z), tentative identification, and quantification (mg/g extract) of the phenolic compounds found (mean ± standard deviation)

Peak	Rt (min)	λ_max (nm)	*[M-H] (m/z)*	MS² (m/z)	Tentative identification	Quantification (mg/g extract)	Reference used for identification
1	4.35	280	461	315(8), 135(28)	Verbasoside	4.35 ± 0.27	[29]
2	5.02	328	487	179(100)	Cistanoside F	3.13 ± 0.09	[29]
3	5.64	358	389	371(23), 345(100), 209(51), 179(10), 121(12)	Theveside	2.43 ± 0.1	[29]
7	11.51	330	639	621(12), 459(23)	β-hydroxy-verbascoside/β-hydroxy-isoverbascoside	3.17 ± 0.04	[29]
8	11.97	330	639	621(15), 459(22)	β-hydroxy-verbascoside/β-hydroxy-isoverbascoside	3.58 ± 0.17	[29]
9	12.69	344	637	351(100), 285(73)	Luteolin 7-O-diglucuronide	17.02 ± 0.06	[30]
10	14.99	314	163	119(100)	p-Coumaric acid	2.69 ± 0.05	[30]
11	15.91	335	621	351(100), 269(24)	Apigenin 7-O-diglucuronide	8.29 ± 0.11	[30]
12	16.66	331	623	461(21), 315(7)	Verbascoside	115.07 ± 0.21	[30]
13	17.99	354	651	351(100), 299(4)	Chrysoeriol 7-O-diglucuronide	3.53 ± 0.03	[30]
14	18.69	331	623	461(19), 315(14)	Isoverbascoside	2.62 ± 0.01	[30]
15	19.23	331	623	461(16), 315(5)	Forsythoside	8.23 ± 0.36	[30]
16	21.23	350	491	315(100), 300(24)	Isorhamnetin 3-O-glucuronide	0.76 ± 0.01	[30]
17	22.56	332	637	491(6), 461(63), 315(14)	Eukovoside	2.06 ± 0.01	[30]
18	22.48	332	651	505(5), 475(27)	Martinoside	2.36 ± 0.06	[30]
Total caffeoyl phenylethanoid glycoside (mg/g extract)						147.00 ± 0.15
Total phenolic acids (mg/g extract)						2.69 ± 0.05
Total flavonoids (mg/g extract)						29.6 ± 0.15
Total phenolic compounds (mg/g extract)						179.29 ± 0.35

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Rt: retention time; λ_max: maximum absorption in the visible region; [M-H]: deprotonated ion; MS²: mass fragmentation