From:  Single nucleotide polymorphisms: rs833061, rs699947, and rs35569394, and the expression of the vascular endothelial growth factor gene in Moroccan patients with lung cancer

Primers’ sequences used for VEGF-A SNPs assessment and VEGF-A, VEGFR-1, and VEGFR-2 expression analyses

AnalysisForward primer 5’-3’Reverse primer 5’-3’Fragment lengthChromosome position
SNPs
-460T/CTGTGCAGACGGCAGTCACCACCCGCTACCAGCCGACTTT588 bp43769749
-2578C/AATAAGGGCCTTAGGACACCAGCTACTTCTCCAGGCTCACA459 bp43768652
-2549I/DATAAGGGCCTTAGGACACCAGCTACTTCTCCAGGCTCACA459 bp43736418
Expression
β2MGAGGCTATCCAGCGTACTCCACGGCAGGCATACTCATCTTTT--
VEGF-AAGGGCAGAATCATCACGAAGTAGGGTCTCGATTGGATGGCA--
VEGFR-1GAAAACGCATAATCTGGGACAGTGCGTGGTGTGCTTATTTGGA--
VEGFR-2GTGATCGGAAATGACACTGGAGCATGTTGGTCACTAACAGAAGCA--
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SNPs: single nucleotide polymorphisms; VEGFR-1: vascular endothelial growth factor receptor; β2M: β2 microglobulin. -: no data