Primers sequences used for PCR amplification
| Gene | Primers sequences (5–3) | Tm (°C) | PCR product size (bp) |
|---|---|---|---|
| β-globin | GH20-F 5'-GAAGAGCCAAGGACAGGTAC-3' | 54 | 268 |
| PC04-R 5'-CAACTTCATCCACGTTCACC-3' | |||
| HPV | MY09-F 5'-CGTCCMARRGGAWACTGATC-3' | 55 | 450 |
| MY11-R 5'-GCMCAGGGWCATAAYAATGG-3' | |||
| GP5+-F 5'-TTTGTTACTGTGGTAGATACTAC-3' | 42 | 150 | |
| GP6+-R 5'-GAAAAATAAACTGTAAATCATATTC-3' | |||
| EBV | EBNA-2 OP-F 5'-GCGGGTGGAGGGAAAGG-3' | 58 | 168 |
| EBNA-2 OP-R 5'-GTCAGCCAAGGGACGCG-3' | |||
| EBNA-2 IP-F 5'-AGGCTGCCCACCCTGAGGAT-3' | 66 | 168 | |
| EBNA-2 IP-R 5'-GCCACCTGGCAGCCCTAAAG-3' | |||
| SV40 | SVTAGP1-F 5'-TTAGCAATTCTGAAGGAAAGTCCTTG-3' | 52 | 126 |
| SVTAGP3-F 5'-ACCTGTTTTGCTCAGAAG-3' | |||
| MMTV | MMTV1-F 5'-TGCGCCTTCCCTGACCAAGGG-3' | 54 | 356 |
| MMTV2-R 5'-GTAACACAGGCAGATGTAGG-3' |
F: forward primer; R: reverse primer; PCR: polymerase chain reaction; Tm: melting temperature; HPV: human papillomavirus; EBV: Epstein-Barr virus; MMTV: mouse mammary tumor virus; SV40: Simian virus 40. M = A + C, R = A + G, W= A + T, Y = C + T