Primers used for detection of β-globin and VDR gene polymorphisms
| Genes | Primers | Sequences | Annealing temperature (°C) | Amplified fragment size (bp) | Reference |
|---|---|---|---|---|---|
| β-globin gene | PC04 | 54 | 268 | [34] | |
| GH20 | |||||
| VDR gene SNP (rs1544410) restricted by BsmI | Forward | 5'-CAACCAAGACTACAAGTACCGCGTCAGTGA-3' | 56 | 825 | [35] |
| Reverse | 5'-AACCAGCGGGAAGAGGTCAAGGG-3' | ||||
| VDR gene SNP (rs7975232)/(rs731236) restricted by ApaI/TaqI | Forward | 5'-CAGAGCATGGACAGGGAGCAA-3' | 66 | 740 | [36] |
| Reverse | 5'-GCAACTCCTCATGGCTGAGGTCTC-3' |
VDR: vitamin D receptor; SNP: single nucleotide polymorphism