Real-time PCR primer sequence
| Gene | Forward | Reverse | Tm (°C) |
|---|---|---|---|
| RUNX2 | GAGGGCACAAGTTCTATCTG | CGCTCCGGCCCACAAATCTC | 60 |
| ALP | GCTGTAAGGACATCGCCTACCA | CCTGGCTTTCTCGTCACTCTCA | 60 |
| BSP | GGCAGTAGTGACTCATCCGAAG | GAAAGTGTGGTATTCTCAGCCTC | 60 |
| COL1A1 | GACATGTTCAGCTTTGTGGACCT | GCCAGCAGATCGGTTCCAG | 60 |
| RANKL | GCCTTTCAAGGAGCTGTGCAAAA | GAGCAAAAGGCTGAGCTTCAAGC | 60 |
| OPG | GGTCTCCTGCTAACTCAGAAAGG | CAGCAAACCTGAAGAATGCCTCC | 60 |
PCR: polymerase chain reaction; ALP: alkaline phosphatase; BSP: bone sialoprotein; RUNX2: Runt-related transcription factor 2; Tm: melting temperature; RANKL: receptor activator of nuclear factor kappa beta ligand; OPG: osteoprotegerin