Myofibrillar ATPase activities, protein content and mRNA levels in control and MI animals with or without ENP and LOS for 5 weeks starting at 3 weeks after induction of MI
| Parameters | Control | MI | MI + ENP | MI + LOS |
|---|---|---|---|---|
| A. MF activities (μmol Pi/mg per h) | ||||
| Ca2+-stimulated ATPase activity | 10.8 ± 0.4 | 7.1 ± 0.4 | 8.3 ± 0.65 | 7.9 ± 0.52 |
| Mg2+-ATPase activity | 3.6 ± 0.4 | 3.8 ± 0.45 | 3.2 ± 0.3 | 3.2 ± 0.3 |
| B. Myosin heavy chain content (% of total) | ||||
| Total MHC | 100 | 96.2 ± 4.9 | 100.0 ± 4.6 | 96.0 ± 9.8 |
| α-MHC | 93.5 ± 2.4 | 71.0 ± 1.8 | 83.6 ± 1.3 | 85.4 ± 1.6 |
| β-MHC | 6.5 ± 0.3 | 29.0 ± 1.5 | 17.6 ± 1.2 | 13.8 ± 0.4 |
| C. Myosin heavy chain mRNA levels (Relative intensity) | ||||
| α-MHC | 92.25 ± 0.25 | 71.25 ± 1.25 | 81.90 ± 1.9 | 82.75 ± 2.5 |
| β-MHC | 7.50 ± 0.25 | 30.00 ± 1.25 | 19.75 ± 1.75 | 15.62 ± 0.12 |
Values are mean ± SE of 7 animals in each group. MLC: myosin light chain. Data are based on the analysis of the information in Figures 1, 2 and 3 in our paper Wang et al. Biochim Biophys Acta. 2004;1690:177–84 [119]. *P < 0.05 compared with control; †P < 0.05 compared with MI group