Exploration of Medicine

Table 4

Sarcoplasmic reticular Ca²⁺ uptake and Ca²⁺ release activities, protein content and mRNA levels in control and MI animals with or without ENP and LOS treatments for 4 weeks starting at 3 weeks after induction of MI

Parameters	Control	MI	MI+ENP	MI+LOS
A. Ca²⁺ uptake activity (nmol/mg per 2 min)	110 ± 8.15	63.5 ± 4.8^*	92 ± 6.3^†	82 ± 4.8^†
B. Ca²⁺ release activity (nmol/mg per 15 s)	8.25 ± 0.32	4.95 ± 0.43^*	7.60 ± 0.37^†	7.20 ± 0.24^†
C. Protein content (% of control)
Ryanodine receptor	100	58.5 ± 1.5^*	85.9 ± 3.1^†	83.8 ± 2.2^†
Ca²⁺-stimulated ATPase	100	50.5 ± 2.5^*	78.2 ± 1.8^†	67 ± 2.5^†
Calsequestrin	100	105.2 ± 4.8	98.3 ± 4.7	96.9 ± 13.1
Phospholamban	100	69.1 ± 5.9^*	86.6 ± 4.4^†	80.8 ± 8.2^†
D. mRNA levels (% of control)
Ryanodine receptor	100	64.8 ± 5.3^*	84.75 ± 2.6^†	86.8 ± 4.5^†
Ca²⁺-stimulated ATPase	100	73.9 ± 4.1^*	86.7 ± 5.3^†	98.6 ± 6.4^†
Calsequestrin	100	105.4 ± 1.6	97.9 ± 7.1	97.4 ± 4.6
Phospholamban	100	78.3 ± 3.7^*	97.3 ± 5.7^†	92.0 ± 6.5^†

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Values are mean ± SE of 7 animals in each group. Data are based on the analysis of the information in table 5 and figures 3 and 4 in our papers Shao et al. Am J Physiol Circ Physiol. 2005;288:H1674–82 [113] and Guo et al. Mol Cell Biochem. 2003;254:163–72 [118]. ^*P < 0.05 compared with control; ^†P < 0.05 compared with MI group