Exploration of Medicine

Table 7

Cardiac performance, status of RAS, oxidative stress and subcellular activities in 8 weeks diabetic rats with or without ENP and LOS treatments

Parameters	Control	Diabetic	Diabetic + ENP	Diabetic + LOS
A. Cardiac function parameters
LVSP (mm Hg)	140 ± 12.1	85 ± 8.4^*	119 ± 7.6^†	116 ± 6.9^†
+ dP/dt (mm Hg/sec)	8856 ± 815	5380 ± 621^*	7176 ± 702^†	7044 ± 516^†
B. RAS activities
Plasma ANG II (fmol/ml)	7.2 ± 0.8	7.4 ± 0.5	6.4 ± 0.6	10.9 ± 0.4^†
Plasma ACE activity (nmol/min per ml)	48 ± 2.5	52 ± 3.1	49 ± 3.5	57 ± 2.6
LV ACE activity (nmol/min per mg)	0.57 ± 0.03	0.89 ± 0.04^*	0.56 ± 0.3^†	0.79 ± 0.03
C. Oxidative stress levels
LV GSH/GSSG Ratio	6.8 ± 0.4	2.5 ± 0.2^*	4.3 ± 0.5^†	4.8 ± 0.2^†
MDA content (nmol/mg tissue lipids)	3.8 ± 0.13	7.1 ± 0.49^*	4.9 ± 0.46^†	5.4 ± 0.48^†
D. Myosin ATPase activities
Mg²⁺-ATPase (nmol Pi/mg per 5 min)	1.2 ± 0.03	0.78 ± 0.07^*	1.06 ± 0.05^†	1.00 ± 0.06^†
Ca²⁺-ATPase (nmol Pi/mg per 5 min)	6.22 ± 0.18	3.14 ± 0.21^*	4.91 ± 0.23^†	4.86±0.28^†
E. SL activities
Na⁺-K⁺-ATPase (μmol Pi/mg per h)	23.2 ± 3.5	13.1 ± 1.8^*	18.2 ± 1.6^†	18.3 ± 1.5^†
Na⁺-Ca²⁺-exchanger (nmol Ca²⁺/mg per 10s)	21.3 ± 1.2	12.1 ± 0.9^*	17.3 ± 1.2^†	16.1 ± 1.5^†
F. SR activities
Ca²⁺-stimulated ATPase (nmol Pi/mg per 5 min)	165 ± 7	115 ± 10^*	154 ± 5^†	153 ± 6^†
Ca²⁺-uptake (nmol Ca²⁺/mg per 2 min)	62.7 ± 2.3	36.5 ± 3.1^*	50.3 ± 2.1^†	53.4 ± 2.7^†

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Values are mean SE of 6 to 8 animals in each group. Data are based on the analysis of the information in Tables 1 and 2 in our paper Liu et al. Ann. N.Y. Acad Sci. 2006;1084:141–54 [174] and Figures 1A, 3B, 5A and 6A in our paper Machackova et al. Mol Cell Biochem. 2004;261:271–8 [175]. ^*Significantly different (P < 0.05) from control; ^†Significantly different (P < 0.05) from untreated diabetic