Association of placental OPRM1 methylation levels with NOWS outcomes
| CpG site | Unadjusted results | P-value univariate | Adjusted results | P-value multivariate |
|---|---|---|---|---|
| NOWS pharmacologic treatment: OR (95% CI) | ||||
| -60 | 1.00 (0.95–1.06) | 0.92 | 1.01 (0.95–1.07) | 0.83 |
| -50 | 0.98 (0.89–1.07) | 0.62 | 0.97 (0.87–1.08) | 0.52 |
| -32 | 1.01 (0.92–1.11) | 0.86 | 1.02 (0.90–1.16) | 0.75 |
| -25 | 0.97 (0.87–1.08) | 0.53 | 0.96 (0.84–1.09) | 0.50 |
| -18 | 0.99 (0.88–1.11) | 0.84 | 1.04 (0.90–1.19) | 0.62 |
| -14 | 0.99 (0.93–1.05) | 0.67 | 0.99 (0.93–1.06) | 0.76 |
| Average | 0.99 (0.89–1.09) | 0.78 | 0.99 (0.89–1.11) | 0.90 |
| LOS (days) due to NOWS: beta (95% CI) | ||||
| -60 | –0.001 (–0.19, 0.19) | 0.99 | –0.02 (–0.20, 0.17) | 0.85 |
| -50 | 0.03 (–0.30, 0.37) | 0.84 | 0.02 (–0.29, 0.34) | 0.89 |
| -32 | 0.007 (–0.33, 0.34) | 0.99 | –0.06 (–0.39, 0.27) | 0.72 |
| -25 | –0.04 (–0.41, 0.32) | 0.81 | –0.08 (–0.43, 0.20) | 0.66 |
| -18 | –0.16 (–0.58, 0.27) | 0.47 | –0.05 (–0.47, 0.38) | 0.83 |
| -14 | 0.06 (–0.14, 0.26) | 0.55 | 0.07 (–0.13, 0.27) | 0.48 |
| Average | 0.01 (–0.33, 0.36) | 0.94 | 0.0009 (–0.33, 0.33) | 0.99 |
| Morphine treatment days: beta (95% CI) | ||||
| -60 | 1.00 (0.94, 1.07) | 0.92 | 1.00 (0.94, 1.07) | 0.97 |
| -50 | 0.96 (0.86, 1.07) | 0.47 | 0.94 (0.84, 1.06) | 0.35 |
| -32 | 1.07 (0.96, 1.18) | 0.22 | 1.05 (0.95, 1.17) | 0.34 |
| -25 | 1.05 (0.93, 1.19) | 0.43 | 1.03 (0.92, 1.17) | 0.59 |
| -18 | 0.99 (0.87, 1.14) | 0.98 | 0.99 (0.86, 1.14) | 0.90 |
| -14 | 1.01 (0.94, 1.08) | 0.83 | 1.00 (0.94, 1.07) | 0.98 |
| Average | 1.02 (0.97, 1.13) | 0.77 | 1.01 (0.90, 1.12) | 0.92 |
| Two medications to treat NOWS: OR (95% CI) | ||||
| -60 | –0.06 (–0.27, 0.14) | 0.54 | –0.05 (–0.26, 0.16) | 0.62 |
| -50 | 0.06 (–0.29, 0.42) | 0.72 | 0.07 (–0.29, 0.44) | 0.69 |
| -32 | –0.13 (–0.48, 0.21) | 0.44 | –0.14 (–0.51, 0.23) | 0.45 |
| -25 | –0.06 (–0.42, 0.30) | 0.73 | –0.06 (–0.44, 0.32) | 0.74 |
| -18 | –0.16 (–0.64, 0.32) | 0.51 | –0.17 (–0.69, 0.34) | 0.50 |
| -14 | 0.05 (–0.17, 0.28) | 0.63 | 0.07 (–0.16, 0.29) | 0.56 |
| Average | –0.05 (–0.44, 0.33) | 0.78 | –0.04 (–0.44, 0.36) | 0.85 |
NOWS pharmacologic treatment adjusted for smoking and maternal opioid; LOS and morphine treatment days adjusted for illicit drugs, smoking, and maternal opioid; two medications for NOWS adjusted for smoking. CI: confidence interval; OR: odds ratio