From:  Liquid biopsy and glioblastoma

Current clinical studies using liquid biopsy in glioblastomas

YearStudyTumorName/MethodOutcome measures
2020–2024NCT04201873 [72]
Interventional phase I		40 Recurrent GBMRNA-seq and nanoString IO360Other outcome/measures: gene expression signature from peripheral blood before/after treatment
2020–2025NCT04274283 [73]
Observational		1,000 Glioma II–IVTessa Jowell BRAIN MATRIX, whole genome and epigenomic classificationMatching tumor and blood, but not CSF, for targetable mutations
2021–2024NCT04931732 [74]
CircTeloDIAG
Observational		150 MRI suspected: glioma II–IV, incl. GBMCicTeloDIAG
3 oncogenic pathways:
IDH
TERT
ATRX		Validate liquid biopsy for glioma II–IV; blood ctDNA (also from CTCs) for diagnosis and monitoring of relapse
2021–2025NCT05099068 [75]
Interventional		500 Advanced/metastatic tumors, GBM, CLLPLANET sequential liquid biopsy, WES, RNA-seqMonitoring changes in the genetic profile of GBM after chemotherapy
2022Pagès et al. [76]258 CNS tumors, incl. HGG (no adult GMB)ULP-WGSctDNA detection: CSF > blood, not in urine. Liquid biopsy is useful for high-grade tumors
2022NCT04539431 [77]
Observational		220 GliomaSensiScreen gliomaValidation of cheaper, more sensitive PCR-platform for liquid biopsy (blood and CSF), comparison with tissue
2022–2023NCT05383872 [78]
Diagnostic		57 GBMMicrobubble resonator (Exablate Model 4000)BBBD or liquid biopsy expected to increase cfDNA
2022–2024NCT05281731 [87]
Interventional		20 GBMSonobiopsy device testing, blood ctDNA, deep sequencingMatching mutations post-sonobiopsy and tumor tissue
2022NCT05133154 [79]
Exploratory		50 Participants, DLGG (30 low-grade, 10 high-grade glioma, 10 controls)Liquid biopsy in low-grade glioma, CTC, TEPSearch for CTCs (> 0), TEP in blood, evaluation of blood-based biomarkers (IDH, 1p19q, ATRX) for diagnosis and monitoring
2022–2023NCT05536024 [80]
Observational		500 GliomaLiquid biopsy, deep learning MRIPrediction of glioma grading and molecular subtype
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IO360: Immuno-Oncology 360°; HGG: high-grade glioma; ULP-WGS: ultra-low-pass whole-genome sequencing