From:  Liquid biopsy: an examination of platelet RNA obtained from head and neck squamous cell carcinoma patients for predictive molecular tumor markers

Primer characteristics

Gene name/NCBI accession NREfficiency test mRNAAmplicon size (bp)Primer forwardPrimer reverse
GAPDH/NM_001357943.22.00/universal RNA115GCTCTCTGCTCCTCCTGTTCACGACCAAATCCGTTGACTC
UBC/NM_021009.72.00/universal RNA105CTGATCAGCAGAGGTTGATCTTTGACGGAGTACCAGGTGCAAG
HPRT1/NM_000194.31.98/universal RNA94TGACACTGGCAAAACAATGCAGGTCCTTTTCACCAGCAAGCT
PGK1/NM_000291.42.00/universal RNA181GGACAATGGAGCCAAGTCGGTGGGTTGGCACAGGCTTTCT
COL1A1/NM_000088.41.96/universal RNA185TGGTTCGTGACCGTGACCTCCAGGTTGCAGCCTTGGTTGG
ZNF750/NM_024702.32.00/UDSCC583GCACAGAATGCCTACCTGCCAGGAGGGTCTCCGTTCACAAC
MAGEA6/NM_005363.52.00/universal RNA182TCGGGGATCCCAAGAAGCTGAATGCGAGGTCCTCCACTGA
Ly6D/NM_003695.31.89/UDSCC577GCCAGCTCTCGCTTCTGCAATCCGCACAGTCCTTCTTCACC
WNT5a/XM_011534089.22.00/universal RNA66AGCGGGTTCCTGAGTGAATCCCCTGACCCCTCTCTAGTT
FAT1/NM_005245.42.00/universal RNA153ATCCTGCAACCGGCTCTCTCCGTGGTCATTCGTGTCGCTG
KRT5/NM_000424.42.00/universal RNA91GCAGATCAAGACCCTCAACAATCCACTTGGTGTCCAGAACCT
EGFR/NM_005228.52.00/universal RNA200TCCTCATTGCCCTCAACACAGTGGCAGGGTTGTTGCTGAACC
PDPN/NM_198389.21.95/universal RNA176CTCAAACGTGGCCACCAGTCGCCTTCCCGACATTTTTCGCA
CDH11/NM_001330576.22.00/universal RNA195ACACGGCCAATGGACCAAGATGCTACTCATGGGCGGGATG
CA12/NM_206925.32.00/universal RNA173AGTTTTCCGAAACCCCGTGCGGATGATGCCTTGGGAGAAGGA
RBP1/NM_001130992.32.00/universal RNA161GCTCCAGTCACTCCCCGAAACACCGTCCTGCACGATCTCT
PLA2G4E/XM_011521238.2, XM_011521239.2, NM_001206670.1, XM_011521238.2

2.00/universal RNA

UDSCC5

76, 143, 193, 197GCCAGCTACATCACCGGTCT, TGTCAACTCCAGCTACCCGC, CAGAGCCCACAAACGGATGA, TGCAGAAGCGGAAGGTCGTTGGAGGACCAGTCAGGGTCA, TGGGGAAGGGGATGTTCTGC, CAGCCTGCCGGACATTTTTCA, ACCGGTGATGTAGCTGGCAC
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NR: number; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; UBC: ubiquitin C; HPRT1: hypoxanthine phosphoribosyltransferase 1; PGK1: phosphoglycerate kinase 1; COL1A1: collagen type I alpha 1 chain; ZNF750: zinc finger protein 750; MAGEA6: MAGE family member A6; Ly6D: lymphocyte antigen 6 family member D; WNT5a: Wnt family member 5a; FAT1: FAT atypical cadherin 1; KRT5: keratin 5; PDPN: podoplanin; CDH11: cadherin 11; CA12: carbonic anhydrase 12; RBP1: retinol binding protein 1; PLA2G4E: phospholipase A2 group IVE