RNA

• Can be used for either short-term or long-term silencing effect

• Supports both synthetic or genetic delivery methods to target cell

• Cost-effective and easy to use

• Can be used for inducible genetic knockdown

• High off-target effect

• Mostly provide partial knockdown of target

• Activation of compensatory pathway

• Not effective for proteins with long half-life

DNA

• Gene editing with a complete knockout of a gene or a gene segment

• Precision

• Irreversible process

• High feasibility

• Time-consuming

• Essential gene knockouts are lethal

• Difficult to aim larger proteins

• Off-target effect generating indels

Protein

• Directly eliminates target protein

• Can target essential or undruggable proteins

• Rapid, hence low chances of compensatory activation

• Less chance of immune response

• Tedious design and synthesis challenge

• Expensive