PROTAC

Advantages:

• Can be used for undruggable molecules

• Targeted degradation

• Low toxicity

• Avoids development of drug resistance

Limitations:

• Requires prior chemical synthesis

• Off-target activity

• Only for intracellular protein

Intrabodies

Advantages:

• More specific

• Can target splice variants or mutant forms

• For both membranous and intracellular proteins

Limitations:

• Time-consuming

• Off-target effects

• Immunogenicity

• Needs modifications for netter delivery and stability

TRIM-Away

Advantages:

• High specificity

• Rapid and easy to use

• Does not require prior chemical synthesis

• Can target specific PTMs

Limitations

• Require highly-specific antibodies

• Lack of in vivo validation

• Requires exogenous delivery of antibody