Culture conditions for the generation of adipocyte CM
| CM conditions | Adipocytes | Matrigel-based ECM | Media type |
|---|---|---|---|
| 1 | – | – | DMEM/FBS |
| 2 | – | + | DMEM/FBS |
| 3 | – | – | Supplemented MEGM |
| 4 | – | – | Supplemented MEGM |
| 5 | + | – | DMEM/FBS |
| 6 | + | + | DMEM/FBS |
| 7 | + | – | Supplemented MEGM |
| 8 | + | + | Supplemented MEGM |
–: exclusion of variable in culture; +: addition of variable in culture