Efficiencies, amplicon length and sequences of qPCR primers used for quantification of murine transcripts
| Gene name | Efficiency (%) | Amplicon length | Forward sequence (5’–3’) | Reverse sequence (5’–3’) | NCBI accession |
|---|---|---|---|---|---|
| HSPA9 | 92 | 112 bp | GGGCAAACAAGCAAAGGTCC | TTGCCGTTTTGCTGGCATAC | NM_010481 |
| GNPDA1 | 142 | 170 bp | GGACGCCACCTTGGAACTAA | GAATACCACGTCCTGGCACA | NM_011937 |
| ATP6V1E1 | 102 | 87 bp | ATAGCCCAGCAGATGATGCC | TCCTCCTGAAGCTCAGTCCA | NM_007510 |
| LOX | 103 | 165 bp | CAGGAACCGACCTGGATACG | GCCCTATATGCTGAACTGGC | NM_010728 |
| GUSB | 91 | 108 bp | CAGCGGCTGGGCTTTTTA | CGCTTGCCCTCAACCAAGTT | NM_010368 |
NCBI: National Center for Biotechnology Information