Exploration of Targeted Anti-tumor Therapy

Tissue dissociation methods	Advantages	Disadvantages
Enzymatic [8–10]	-Extraction of a sufficient quantity of required cells. -Accessibility and widespread application. -Relatively easy removal of stromal cells and fibroblasts.	Requires the selection of specific enzymes for each type of tumor tissue.
Chemical [11, 12]	-Allows better preservation of phenotypic and genetic characteristics. -Cells divide rapidly when cultivated.	The complexity of the procedure, with the efficiency not fully established.
Mechanic [8–13]	Simplicity and accessibility, with no chemical impact on cells.	-Formation of a large number of non-viable cells. -Release of cell contents with damaged membranes into the external environment, and sample contamination.

Tissue dissociation methods

Advantages

Disadvantages

Enzymatic [8–10]

-Extraction of a sufficient quantity of required cells.
-Accessibility and widespread application.
-Relatively easy removal of stromal cells and fibroblasts.

Requires the selection of specific enzymes for each type of tumor tissue.

Chemical [11, 12]

-Allows better preservation of phenotypic and genetic characteristics.
-Cells divide rapidly when cultivated.

The complexity of the procedure, with the efficiency not fully established.

Mechanic [8–13]

Simplicity and accessibility, with no chemical impact on cells.

-Formation of a large number of non-viable cells.
-Release of cell contents with damaged membranes into the external environment, and sample contamination.