Alterations of frequently mutated genes of colorectal cancer in colorectal cancer cell lines with or without APC and other WNT/β-catenin pathway mutations
| Cell line | TP53 | KRAS | NRAS | BRAF | PIK3CA | APC | RNF43 | CTNNB1 | TCF7L2 |
|---|---|---|---|---|---|---|---|---|---|
| Quadruple mutated | |||||||||
| CL-34 | *S127P, *K382Nfs*40 | WT | WT | *V600E | WT | *T1556Nfs*3, *E418*, R856C | WT | WT | *K468Sfs*23, L200I |
| CW-2 | WT | P140H | WT | WT | P283S | *S1465Wfs*3, *R302*, G470R, A528V, E2737G, I2756V | WT | R582Q | *C469Vfs*8 |
| GP2d | WT | *G12D | WT | T529A | *H1047L | *T1445Lfs*27, L2384I, S2562G, HD | S771T, D628G | D755V | *W156* |
| HCT-15 | *S241F, *X367_splice | *G13D | WT | WT | *E545K, *D549N | *I1417Lfs*2, *R2166*, R727M, K993N, K1561N, E2550Q, I1779M | *G659Vfs*41, L214M | WT | WT |
| KM12 | *H179R, *V73Wfs*50 | WT | WT | A712T, A404Cfs*9 | WT | *N1818Kfs*2, G471E | *G659Vfs*41 | SETD5 fusion | WT |
| LoVo | WT | *G13D | WT | WT | WT | *R1114*, *M1431Cfs*42, R2816Q | WT | *R535Q | *K468Sfs*23 |
| LS411N | *Y126* | WT | WT | *V600E | WT | *T1556Nfs*3, *Q789* | *G659Vfs*41 | WT | WT |
| NCI-H630 | *R342* | WT | WT | WT | WT | *Q1367*, V1173M | WT | WT | *K468Sfs*23 |
| SNU-C2A | *R273C, *R273H, *R273Y | *G12D | HD | WT | D725G | *K2051Efs*9 | *R337*, C275Wfs*143, R389C | HD | *K468Sfs*23 |
| SNU-C4 | *G245S | WT | HD | D22N | *E545G, V71I | *F801Lfs*19, *T1556Lfs*9, H325R | *R225Afs*194 | WT | *K468Sfs*23 |
| SNU-C5 | *R248W, *V218L | WT | WT | *V600E | *H1047R | *N1792Kfs*7 | *G659Vfs*41 | WT | WT |
| APC only mutated | |||||||||
| C75 | *R249S | WT | WT | WT | WT | *Q1204*, *S943Qfs*12, *L1488Ffs*23 | WT | WT | WT |
| C80 | *Q52* | *A146V | WT | WT | WT | *L629* | WT | WT | WT |
| C84 | *R342* | *G12A | WT | WT | WT | *R1450*, *R283*, *R640W | WT | WT | WT |
| CACO2 | *C135F, *E204* | WT | WT | WT | WT | *Q1367* | WT | G245A | WT |
| COLO 201 | WT | WT | WT | *V600E | WT | *T1556Nfs*3 | WT | WT | WT |
| DiFi | *K132R | WT | WT | WT | WT | *E1151*, *E443Afs*16 | WT | WT | WT |
| GEO | WT | *G12A | WT | WT | WT | *E1536*, *C344Vfs*110 | WT | WT | WT |
| HCC2998 | *R213* | *A146T | WT | WT | WT | *R1450*, *L665*, I2167S, S1864Y, N2720K, R168I | WT | WT | WT |
| MDST8 | WT | WT | WT | *V600E, *V600K, *V600M | WT | *T1556Nfs*3 | WT | WT | WT |
| SK-CO-1 | WT | *G12V, AMPL | WT | AMPL | WT | *F1089fs*37 | WT | WT | WT |
| SNU-175 | WT | *A59T | WT | NA | NA | *R232*, *N1455fs*18, *G1499*, A199V | WT | WT | WT |
| SW1116 | *A159D | *G12A | WT | NA | AMPL | *Q264*, *Q1429Hfs*41 | WT | WT | WT |
| SW1417 | *C238Hfs*21 | WT | WT | *V600E, AMPL | NA | *R1450* | WT | WT | WT |
| Triple mutated | |||||||||
| HCC-56 | *R196P | *G12V | WT | WT | WT | WT | WT | WT | *X387_splice |
| HCT 116 | WT | *G13D | WT | WT | *H1047R | WT | *R117Afs*41 | *S45del | Fusion |
| LIM1215 | WT | WT | WT | WT | WT | WT | WT | *T41A, Q177P | NA |
| LS1034 | *G245S, HD | *A146T, AMPL | WT | WT | WT | WT | WT | WT | *R39Gfs*4 |
| NCI-H508 | *R273H | WT | WT | *G596R | *E545K, AMPL | WT | WT | WT | Fusion, HD |
| NCI-H716 | *E224D | R97I | WT | WT | WT | WT | *H472Qfs*30 | WT | WT |
| RKO | WT | WT | WT | *V600E | *H1047R | WT | *G659Vfs*41, H549N | WT | WT |
| SNU-407 | *S90Pfs*33 | *G12D | WT | R726C | *H1047R | WT | WT | *T41A | *K468Sfs*23, A549T, A419V |
| SNU-C2B | *R273Y | *G12D | WT | WT | D725G | WT | *C275Wfs*143 | WT | *K468Sfs*23 |
| Quadruple wild type | |||||||||
| C10 | WT | WT | WT | WT | WT | WT | WT | WT | WT |
| C99 | WT | WT | WT | WT | WT | WT | WT | WT | WT |
| CAR1 | *V272M | WT | WT | WT | WT | WT | WT | WT | WT |
| LS513 | WT | *G12D | WT | E204L, E204V, E204* | WT | WT | WT | WT | WT |
AMPL: amplified; HD: Homodeleted; WT: wild type; NA: not available; APC: adenomatous polyposis coli. * before a mutation denotes oncogenic. Data are from the Cancer Cell Line Encyclopedia (CCLE)