From:  Genomic alterations in the WNT/β-catenin pathway and resistance of colorectal cancer cells to pathway-targeting therapies

 RNA interference (RNAi) in colorectal cancer cell lines with or without APC and other WNT/β-catenin pathway mutations

Cell lineAPCRNF43CTNNB1TCF7L2
Gene effectZ-scoreRankGene effectZ-scoreRankGene effectZ-scoreRankGene effectZ-scoreRank
Quadruple mutated
CL-34–0.58–2.678–0.18–0.571,040–1.87–3.014–0.3–1.12275
CW-2–0.18–0.892,2850.32.77,993–4.71–8.33–0.61–2.66354
GP2d–0.82–3.784–0.15–0.43,379–2.06–3.367–0.16–0.413,310
HCT-150.050.235,480–0.010.556,368–1.94–3.14970.060.716,728
KM12–0.28–1.3790–0.2–0.672,741–1.8–2.8928–0.11–0.175,904
LoVo–0.42–1.93254–0.19–0.613,380–1.66–2.6345–0.14–0.35,135
LS411N–0.51–2.35159–0.02–0.528,767–2.18–3.6210.020.518,707
SNU-C2A0.542.4612,975–0.060.248,690–0.170.158,195–0.28–12,370
SNU-C4–0.38–1.79101–0.16–0.461,606–2.04–3.3416–0.27–0.94550
APC only mutated
CACO20.080.3410,937–0.16–0.416,307–0.91–1.232,2200.110.9613,729
COLO 201–0.18–0.852,3240.0090.735,956–2–3.2107–1.35–6.345
MDST80.080.364,4530.071.176,420–0.64–0.731,816–0.16–0.432,428
SK-CO-1–0.72–3.31280.040.9911,450–1.65–2.664–0.47–1.97177
SW1417–0.18–0.861,711–0.19–0.662,3230.090.659,553–0.3–1.121,075
Triple mutated
HCT 116–0.05–0.274,669–0.2–0.692,279–0.56–0.582,766–0.28–0.991,297
NCI-H508–0.11–0.533,510–0.26–1.081,563–1.13–1.64509–0.72–3.2310
NCI-H7160.050.237,7440.04111,047–0.120.237,7580.10.9210,816
RKO0.070.298,335–0.15–0.363,192–0.050.368,9620.030.5610,424
SNU-4070.20.896,985–0.17–0.483,041–2.84–4.837–0.68–3.0368
Quadruple wild type
LS513–0.28–1.321,188–0.020.478,506–1.63–2.57107–0.63–2.7566
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Data were from project Achilles, Drive, Marcotte DEMETER2. The Z-score was computed as the gene effect minus the mean across cell line models divided by the standard deviation (SD). Not all cell lines of the original cohorts had data available in the RNAi arrays. APC: adenomatous polyposis coli