From:  Implementation of an NGS panel for clinical practice in paraffin-embedded tissue samples from locally advanced and metastatic melanoma patients

Concordance of BRAF mutational status established by RT-PCR and NGS methods

RT-PCRTotal
BRAF mutatedBRAF wild-type
NGSBRAF mutated12214
BRAF wild-type01515
Total121729
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Kappa = 0.89, 95% confidence interval (CI) 0.68 ± 1.05. Concordance samples are in bold.