Comparison of retention time of DAR species in RP-HPLC
| DAR species | Trastuzumab-MCC-maytansinoid | Trastuzumab-MC-VC-PAB-MMAE |
|---|---|---|
| LC | 7.0 min | 7.0 min |
| LC + drug-linker | 8.2 min | 9.6 min |
| HC | 10.2 min | 10.2 min |
| HC + drug-linker | 11.0 min | 12.1 min |
| HC + 2 drug-linkers | 13.1 min | 15.4 min |
| HC + 3 drug-linkers | 14.4 min | 18.0 min |
LC: antibody light chain; LC + drug-linker: LC conjugated with one drug-linker; HC + drug-linker: HC conjugated with one drug-linker