Quantification of fatty acids by using various analytical techniques
| Authors name | Disease condition analysed | Metabolites altered | Analytical technique | Healthy vs. NASH patients |
|---|---|---|---|---|
| Masoodi et al. [185] | MASLD and MASH | Amino acids, fatty acids, triglycerides, phospholipids, and bile acids | Metabolomics and lipidomic by MS | Altered amino acid metabolism, and lipid disturbances |
| Kartsoli et al. [186] | MASLD | Liver lipid species | Lipidomic approach by LC-MS/MS | Impact of liver lipid species on MASH development and progression |
| Garcia-Jaramillo et al. [187] | MASH | Hepatic neutral and membrane lipids | Lipidomic analysis by UPLC-TOF-MS/MS | Profound alterations in lipid composition |
| Wang et al. [188] | MASLD and MASH | The main types of lipids are glycerides, glycerophospholipids, sphingolipids, fatty acyl lipids, and sterol lipids. | Lipidomic profiling by UPLC-MS/MS | Differential lipid profiles in urine samples |
| GC-FID | ||||
| Piras et al. [189] | MASLD | Circulating lipid biomarkers | LC-MS | Potential biomarkers for MASLD |
| Tan et al. [190] | MASLD and MASH | Circulating fatty acids, triglycerides, phospholipids, and bile acids | Metabolomics and lipidomics by imaging particle detector | Altered metabolic pathways |
| Kalopitas et al. [191] | MASLD and MASH | Potential differences in plasma lipids | Lipidomic profiling by LC-MS and GC-MS | Comparison in MASH, MASLD, and heathy |
| Zhu et al. [192] | NAFL and NASH | Urinary extracellular vesicles | Lipidomic analysis by mass-based approach | Lipidomic changes as potential markers |
| Gaggini et al. [193] | NAFLD | Circulating lipids | Metabolomics by mass-based approach | Links between lipids and NAFLD |
NASH: non-alcoholic steatohepatitis; MASLD: metabolic dysfunction-associated steatotic liver disease; LC-MS: liquid chromatography-mass spectrometry; UPLC-TOF-MS: ultra-performance liquid chromatography time-of-flight mass spectrometry; UPLC-MS: ultra-performance liquid chromatography-mass spectrometry; GC-FID: gas chromatography flame ionization detector; GC-MS: gas chromatography-mass spectrometry; NAFL: non-alcoholic fatty liver; NAFLD: non-alcoholic fatty liver disease