The extraction, separation, and purification of dietary proteins from a variety of food sources are crucial for their targeted use in food applications. To achieve this, proteins should be effectively separated from non-protein components such as cell wall structures, polysaccharides, and lipids. Traditional protein purification methods can be time-consuming, highlighting the need for automated, cost-effective, and sustainable alternatives. This comprehensive review critically assesses various protein purification instruments from an analytical perspective, weighing their advantages and disadvantages. The methods under evaluation include ultrafiltration, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), fast protein liquid chromatography (FPLC), high-performance liquid chromatography (HPLC), ultra performance liquid chromatography (UPLC), and microfluidic chips. Among these, FPLC stands out as an affordable and efficient technique that allows for high protein recovery. However, HPLC and UPLC provide faster results but may denature proteins, leading to lower recovery rates. Ultrafiltration is a cost-effective and straightforward method that doesn’t require complex equipment. Microchip-based approaches are emerging as innovative techniques for rapidly analyzing small samples. While SDS-PAGE is user-friendly, it denatures proteins, particularly those linked to other biomolecules. The choice of the most appropriate instrument depends on factors such as cost, energy efficiency, processing time, the characteristics of the target protein, desired outcomes, protein recovery, and resource availability. By critically examining these analytical instruments for protein purification, this review aims to assist researchers and practitioners in selecting the most suitable method for their specific needs, ultimately promoting efficient and successful protein purification endeavors in the field of food science and technology.

The objective of this work was to compile data for the characterization of pistachio’s chemical composition and to analyze the benefits of their consumption in the diet. Pistachio edible seed is cultivated mainly in America, Mediterranean countries and Middle East. The geographical precedence may affect its mineral content as well as its lipidic profile and it may also influence the content of bioactive compounds. Pistachio presents a high proportion of vitamins, carotenoids, polyphenols, and flavonoids that have been associated with pistachio health benefits such as its antioxidant and anti-inflammatory activities. Pistachio intake would reduce glycemic index and control Type-2 Diabetes Mellitus. Clinical studies have also indicated that the presence of phytosterols, monounsaturated fatty acids (MUFAs) and dietary fiber from pistachio grains may reduce the risk of cardiovascular diseases (CVDs). Furthermore, the main wastes of pistachio industry [pistachio green hull (PGH) and pistachio shell (PS)] could be also considered a good source of bioactive compounds. Recent studies showed that the encapsulation of these nutraceutical compounds of PGH may be a green strategy for manufacture high-value foods within the framework of circular economy. Moreover, PS can be considered a good source of cellulose nanocrystals (CNC) that may be used for encapsulation and stabilization of oil-water emulsions.

This paper reviews the nutritional quality and safety of edible farmed insects from the point of view of the Czech-Slovenian bilateral project: Quality, Safety and Authenticity of Insect Protein-based Food and Feed (INPROFF). Insects as a sustainable source of dietary protein for animal feed and even humans, when integrated into the European agrifood system, could offer a solution to Europe’s feed protein deficit and help alleviate environmental pressure from increasing protein demand, such as declining availability of land, water, marine and energy resources, the overuse of pesticides and reduced biodiversity. However, despite a growing interest in the European Union (EU) in farming edible insects, many economic, scientific, technological, and social barriers remain. In response, Slovenia, represented by the Jožef Stefan Institute, the Biotechnical Faculty of the University of Ljubljana, and Jata Emona d.o.o. (the leading supplier of feedstuffs for the Slovenian market), a country with no history of insect rearing but an interest in alternative protein, joined with the Czech University of Life Sciences Prague, representing the Czech Republic—a country with a history of insect rearing and research into edible insects—to establish INPROFF a three-year bilateral project that aims to close the knowledge gap regarding the quality and safety of insect-based products and boost the farmed insect food and feed value chain. Specifically, it comprises three thematic pillars: (P1) nutritional enhancement, (P2) safety and quality and (P3) authenticity, traceability, and consumer acceptance. The paper also discusses the gaps in the metrological challenges of analysing insects, which will be critical for ensuring safety, quality, and sustainability. The paper finds that although much work has been done, many exciting avenues remain for new research.

Over the past few decades, there has been a major increase in type 2 diabetes (T2D) prevalence, a long-term medical condition in which your body doesn't use insulin properly in most regions of the world. After adjusting for the impact of aging populations, diabetes prevalence in adults (85–95% T2D) almost doubled between 1980 and 2020 worldwide. Increases were more pronounced in low- and middle-income countries and in men compared to women. The aim of this study is to evaluate the performance of a very low-calories ketogenic diet (VLCKD) as an effective nutritional approach for both TD2 and obesity. A ketogenic diet (KD) positively affects blood glucose levels, body weight, glycosylated hemoglobin, neurological disorders, and plasma lipid profiles. We combined ClinicalTrials.gov data and data from PubMed from 2020 to 2022. Only published papers that met the requirements of reporting clinical trials investigating an adult sample of T2D obese patients were included. The review shows the beneficial therapeutic value of a VLCKD in the management of T2D and long-term obesity and its capacity to help achieve disease remission. Evidence from the literature underlines the need to redefine guidelines to offer a dietary and low-carb option to combat insulin resistance (IR) and related diseases.

Food samples require extensive sample preparations for instrumental analyses due to the complex matrices involved. Food safety regulatory agencies also require sample preparation procedures that are accurate, sensitive, robust, and, above all, fast, to handle the requirements for determining the safety of the massive amounts of foods and food products needed for human, pet and livestock consumption. There is also an inseparable interconnection between environmental, agricultural, forensic, cosmetic and industrial analytical chemistry involved in this requirement, and advances in analytical methodology are simultaneously applicable to all of these realms. As a response to these needs, the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was developed to provide multiclass analysis of agricultural products, and remains the basis for regulatory procedures for large scale analyses of food samples containing a wide variety of possible contaminants. However, since QuEChERS does not enhance analyte concentrations during sample preparation of these complex samples, the methodology also requires very expensive, very sensitive final analytical instrumentation, requiring highly trained personnel and continual maintenance. Smaller regulatory and field laboratories may also need sample preparation procedures for only a limited number of specific pesticides, metals, polycyclic aromatic hydrocarbons (PAHs) or other contaminants, requiring much less expensive and labor-intensive preparations and instrumentation. This is the role of liquid phase microextraction (LPME) in food sample preparation and analysis. LPME, individually or in combination with other sample preparation procedures, such as QuEChERS or traditional techniques such as solid phase extraction (SPE), can meet the requirements for sensitive and accurate analyses of specific analytes found in complex matrices, providing not only cleanup, but concentration of sample extracts, allowing the use of greener, less expensive and low maintenance final determination analytical instrumentation. Crucial review and application publications are tabulated to allow analysts easier access to appropriate publications to use this information for developing new or improved and greener validated methods for plant and animal food analyses.