Contents
Special Issue Topic

Metrological Aspects in the Analysis of Nutrients, Functional Compounds, Additives and Contaminants in Food and Feed

Submission Deadline: November 01, 2025

Guest Editors

Maria Z. Tsimidou E-Mail

Professor Emerita of Food Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece

Research Keywords: nutrients; functional compounds; foo(ee)d additives; method validation; metabolomics

Nives Ogrinc E-Mail

Professor of Ecotechnology, Jožef Stefan International Postgraduate School and Jožef Stefan Institute, Ljubljana, Slovenia

Research Keywords: stable isotopes; trace and toxic element analysis and speciation; volatile organic compounds; metrology

Claudia Zoani E-Mail

Coordinator of METROFOOD-RI, Researcher ENEA, C.R. Casaccia, Rome - Italy

Research Keywords: elemental analysis; emerging contaminants; metrology; research infrastructures; reference materials

About the Special lssue

Dear Colleagues,

We accepted with pleasure the invitation made by Prof. Alejandro Cifuentes and Prof. Elena Ibáñez to be the guest editors of a Special Issue for the open access, bimonthly published and multidisciplinary journal Exploration of Foods and Foodomics that aims at publishing papers of the highest quality and significance in all areas of Foodomics.

We know and collaborate with each other for many years within (and beyond) the frame of METROFOOD-RI, the European Research Infrastructure that set as a priority the high – level metrology services in food and nutrition for the enhancement of food quality and safety. Our common code in scientific actions is to support the practical realisation of the “One Health” approach in foo(ee)d analysis in the whole agroecosystem and in the whole food chain. We fully acknowledge the complexity of foo(ee)d systems in terms of their physical, chemical, nutritional, biological composition and properties and, thus, all aspects in foo(ee)d analysis are welcome.

The thematic area proposed is related to the everyday concern of each food and feed analyst as to how confidence in the analytical data produced in accredited and non-accredited laboratories is secured. Metrology represents the essential basis to make data reliable, providing objectivity and impartiality, supporting harmonisation, and ensuring the comparability of measurement results including reference material production and characterization. Its application to foo(ee)d and nutrition represents an engine to promote research and innovation, infrastructures’ operation, technology advancements, cooperation in global trade, economy and strengthening citizen trust. The application of metrological concepts is essential also with reference to digitalisation and digital transformation when measurements are involved.

New methods, new instrumentation, new materials, new software demand a continuous updating of analytical protocols for an easier and trustful data production and communication. Non-targeted and targeted analytical protocols present different characteristics that have to be addressed from the metrological point of view. Automation, device development and application are ongoing challenging areas of analytical practice that has to also adopt metrological aspects. Data transferability and building of data banks point out to metrology as a prerequisite.

Keywords: food and feed analysis; metrological traceability; analytical methods; sensors and portable devices; method validation; reference materials; nutraceutical and functional foods; emerging contaminants; nutrients; functional compounds; additives and contaminants

Published Articles

Open Access Commentary
How can a European research infrastructure promote metrological aspects of food/feed and nutrition analysis?
Research infrastructures (RIs) are central to European Union (EU) policies for defragmentation of the research and innovation in all fields of science and technology providing resources, expertise,
Published: February 10, 2025 Explor Foods Foodomics. 2025;3:101071
355 88 0
Open Access Perspective
From data to nutrition: the impact of computing infrastructure and artificial intelligence
This article explores the significant impact that artificial intelligence (AI) could have on food safety and nutrition, with a specific focus on the use of machine learning and neural networks for d
Published: December 03, 2024 Explor Foods Foodomics. 2024;2:810–829
1681 170 1
Open Access Original Article
Detection of pharmaceuticals and their transformation products in seawaters using off-line solid phase extraction and liquid chromatography-high resolution mass spectrometry
Aim: Fast urbanization of free land, industrial progress, and improvement of human life quality, have led to increased consumption of different chemical substances recognized as emerging contaminants (ECs) that are chemicals posing potential risks to the environment and human health, but are not yet subjected to regulatory criteria. Pharmaceuticals consumed by humans are continuously discharged into aquatic environments through urban effluents and numerous classes of them have been widely detected in surface waters. We have to highlight that pharmaceuticals, deposited in the aquatic environment end up in human body through the consumption of marine organisms. Consequently, seawater can be considered as feed. Aim of the study is the development, optimization and validation of a multi-residue analytical method concerning the detection of pharmaceuticals in seawaters.
Published: November 29, 2024 Explor Foods Foodomics. 2024;2:767–787
844 146 0
Open Access Original Article
Exploring the potential of triticale lines for bioethanol production
Aim: Triticale is a well adaptable crop, tolerant of disease and abiotic stresses, and able to grow with good yields even in poor soil, thus representing a good choice to develop a new industrial
Published: October 21, 2024 Explor Foods Foodomics. 2024;2:613–625
899 121 0
Open Access Original Article
In-tube extraction dynamic headspace coupled to gas chromatography-mass spectrometry for the sensitive analysis of volatile compounds in aqueous samples
Aim: Volatile organic compounds (VOCs) are often human-made contaminants used and generated in the manufacturing of numerous products, presenting notable environmental and health hazards. Therefo
Published: October 12, 2024 Explor Foods Foodomics. 2024;2:599–612
1204 111 0
Open Access Review
Analytical aspects of metagenomic and volatilomic approaches that advance table olive integrity research
Table olives are one of the most widespread fermented foods in the Mediterranean area, presenting an exponential increase in global consumption in the latest years. As a fermented product, its micro
Published: September 26, 2024 Explor Foods Foodomics. 2024;2:570–598
1049 111 0
Open Access Original Article
Inorganic contaminants and micronutrients in foods consumed by the vegetarian Portuguese population
Aim: The present study aimed to determine the profile of micronutrients and inorganic contaminants in the plant-based and dairy food products most consumed by the Portuguese population. Meth
Published: September 24, 2024 Explor Foods Foodomics. 2024;2:555–569
916 102 0
Open Access Original Article
Exploring the potential of laser photoacoustic spectroscopy (LPAS) for predicting amylose content in rice flour
Aim: Rice, one of the most widely consumed staple foods globally, relies on amylose content for its quality, impacting cooking, digestibility, and health properties. Conventional amylose determin
Published: September 10, 2024 Explor Foods Foodomics. 2024;2:542–554
1350 168 0
Open Access Original Article
Exploring the effect of Pinus halepensis resin quality on the vinification of Retsina by untargeted profile analysis
Aim: Three factors that affect the chemical composition and perceptible aroma of Retsina—a distinguished Greek wine, crafted by infusing resin harvested from the Aleppo pine (Pinus halepensis M
Published: August 29, 2024 Explor Foods Foodomics. 2024;2:497–524
1232 45 1
Open Access Review
Assessing the presence and health risks of potentially toxic metals in food: a comprehensive overview
Food products can contain various substances, including essential nutrients, as well as non-nutritive elements and potentially toxic metals. Metal contaminants have the potential to accumulate withi
Published: August 19, 2024 Explor Foods Foodomics. 2024;2:471–496
1447 26 0
Open Access Review
Quality, safety and authenticity of insect protein-based food and feed: insights from the INPROFF Project
This paper reviews the nutritional quality and safety of edible farmed insects from the point of view of the Czech-Slovenian bilateral project: Quality, Safety and Authenticity of Insect Protein-based Food and Feed (INPROFF). Insects as a sustainable source of dietary protein for animal feed and even humans, when integrated into the European agrifood system, could offer a solution to Europe’s feed protein deficit and help alleviate environmental pressure from increasing protein demand, such as declining availability of land, water, marine and energy resources, the overuse of pesticides and reduced biodiversity. However, despite a growing interest in the European Union (EU) in farming edible insects, many economic, scientific, technological, and social barriers remain. In response, Slovenia, represented by the Jožef Stefan Institute, the Biotechnical Faculty of the University of Ljubljana, and Jata Emona d.o.o. (the leading supplier of feedstuffs for the Slovenian market), a country with no history of insect rearing but an interest in alternative protein, joined with the Czech University of Life Sciences Prague, representing the Czech Republic—a country with a history of insect rearing and research into edible insects—to establish INPROFF a three-year bilateral project that aims to close the knowledge gap regarding the quality and safety of insect-based products and boost the farmed insect food and feed value chain. Specifically, it comprises three thematic pillars: (P1) nutritional enhancement, (P2) safety and quality and (P3) authenticity, traceability, and consumer acceptance. The paper also discusses the gaps in the metrological challenges of analysing insects, which will be critical for ensuring safety, quality, and sustainability. The paper finds that although much work has been done, many exciting avenues remain for new research.
Published: July 24, 2024 Explor Foods Foodomics. 2024;2:339–362
3007 102 1
Open Access Original Article
Metrological aspects of a gas-phase DFT/B3LYP quantum-chemical approach to prioritize radical scavenging activity among a group of olive oil phenols
Aim: A protocol relying on quantum chemical calculations to assist prioritization of phenolic compounds as antioxidants in terms of hydrogen atom donation efficiency is presented. The use of reference compounds, an important metrological issue for a future harmonization and standardization of computational approaches in research is also considered. Methods: A density functional theory (DFT) approach, namely B3LYP/6-311G++(2d,2p)//B3LYP/6-31G in the gas-phase was used for structure optimization, frequency calculation and single point energy (SPE) calculation to obtain the bond dissociation enthalpy (BDE) value of the most active O-H of olive oil phenols. For some of them used as a test set and for comparison, BDE values were calculated using three more approaches, M05-2X in the gas-phase, Becke three-parameter Lee-Yang-Parr (B3LYP) and implicit solvent effects (n-heptane to model bulk oils) with the integral equation formalism version of the polarizable continuum model (IEF-PCM), Minnesota 05 functional with double nonlocal exchange (M05-2X) at a single step using 6-31G+(d) basis set and solvation model density (SMD) as solvation model. Phenol and Trolox were used as reference compounds for ΔBDE calculation. Results: The proposed protocol was faster by 1.35-, 1.6-, and 8.3-fold respectively than the other three and provided almost the same activity trend application to other type of olive oil phenols indicated that prioritization based on ΔBDE values was in accordance with the limited existing experimental findings in bulk oils, and the order of activity was generally in agreement with the structure-antioxidant activity criteria. Conclusions: Present findings highlighted the usefulness of quantum chemical calculations as a tool to screen/prioritize molecules with an established structure saving experimental effort and waste production. The expression of results relatively to phenol and Trolox BDE values, may assist comparisons among research findings and facilitate standardization. Based on the findings hydroxytyrosol and related compounds should be efficient hydrogen atom donors compared to other potent virgin olive oil phenols.
Published: July 18, 2024 Explor Foods Foodomics. 2024;2:326–338
1648 22 0
Open Access Original Article
Building a database for the quality characteristics of the Protected Designation of Origin saffron Krokos Kozanis, considering international trade requirements
Aim: Saffron, a precious spice deriving from the emblematic Crocus sativus L. cultivation, is a traditional product with high socio-economic and cultural value in Greece, especially in the region
Published: June 07, 2024 Explor Foods Foodomics. 2024;2:236–251
1656 28 0